Validating affymetrix chip results with real time pcr
The service includes fragmentation, adding adaptors, size selection, amplification and QC. If the quantity is not a problem, we prefer to have 2.5 ug of total RNA.
But the species are limited to human, mouse and rat.
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Accepting the NEJM cookie is necessary to use the website.This tool helps in alignment of each individual samples to the reference genome and for the calculation of gene expression measures (RPKM) from m RNA sequence data and generation of gene expression profiles .
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